Pipetting and uncertainty of dilutions
Correctly performed dilutions will often give small contribution to the overall uncertainty of the analysis.
Fist we need to have good control of our pipettes - Nordtest has a guide on calibration, verification and routine checks for micropipettes - Nordtest 626.
Second we need to know the uncertainty - a summary of typical uncertanties for different alternatives to dilute aqueous solutions at room temperature are given here. Examples of dilution 1000 times using different equipment are presented.